 หัวเรื่อง:ความสามารถในการก่อให้เกิดโรคของ culture filtrate ของเชื้อรา Pyricularia oryzae Cav. สาเหตุโรคใบไหม้ของข้าว ผู้เขียน: นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์ ,  Petcharat Chuntharathin, Vijai Rukvidhayasartra สื่อสิ่งพิมพ์:pdf AbstractCapability of culture filtrate peoduced by Pyricularia oryzae Cav. to incite blast symptom was conducted on rice variety KDML 105. Five isolates of the fungal pathogen were isolated from rice infected leaves. The result revealed that the symptoms produced by culture filtrate were quite similar to those produced by the fungal pathogen. The capabilities of culture filtrates for inciting disease symptoms were different among 5 isolates of the fungal pathogen. It might be concluded that culture filtrate containing toxic substance which was classified to pathotoxin could played an important role on virulent factor of blast pathogen. The response of rice leaves to culture filtrate was studied. The result indicated that as the increasing of culture filtrate concentrations, sizes and severity of lesions increased. The optimum incubation period for symptom appearance was 5 days after exposing culture filtrate to rice leaves. Rice varieties exhibited different responded to culture filtrate and their levels of response were determined by lesion sizes and lesion types. |
หัวเรื่อง:การตอบสนองของเนื้อเยื้อข้าวและแคลลัสต่อ culture filtrate เชื้อราMagnaporthe grisea (Pyricularia oryzae Cav.) ในขวดเพาะเลี้ยงเนื้อเยื้อ ผู้เขียน:นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์, Petcharat Chuntharathin, Vijai Rukvidhayasartra, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe responses of rice tissue to culture filtrate of the fungus Magnaporthe grisea (Pyricularia oryzae Cav.), the causal agent of rice blast were investigated in vitro. Shoots and calluses of rice variety KDML105 were cultured on medium containing culture filtrate at concentrations of 0:9,1:9, 2:9, 3:9, 4:9 and 5:9 by volume, respectively. The result indicated that rice shoots exhibited significant difference response to different culture filtrate concentrations. Where the concentrations of culture filtrate increased, the height, number and survival of shoots decreased. At the concentration of 2:9, the height and number of shoots were reduced to 50 percent. The increasing of culturing period had an influence on the increasing of height and the number of shoots while the survival of shoots were reduced. Some of brown tissue could reproduce newly emerged shoots after culturing for a longer period.The responses of calluses to culture filtrate were investigated. The results revealed that where the concentrations of culture filtrate were increased, the calluses color became brown, while the diameter of calluses, amount of green spot formations and the number of regenerated shoots were reduced. At the concentration of 2:9, callus diameter was reduced to 50 percent, the callus color was changed to brown 50 percent, the percentage of green spot formations and the regenerated shoots were the highest at 3.6 and 12.5 percent, respectively. The result suggested that culturing period had a significant influence on callus development. On induction medium, while the culturing period was increased, the size of calli, degree of yellowing and green spot formations were increased whereas the color of calli was changed to brown, the number of survival was reduced and the number of regenerated shoots were increased when they were cultured on a regeneration medium. |